《植物生理学报》 2015, 51(11): 1975-1981
通信作者:宋兴舜;E-mail: sfandi@163.com;Tel: 0451-82191755
摘 要:
本实验以拟南芥野生型(WT)及vde基因缺失突变体植株(NPQ1-2)为材料, 分别研究了它们在PEG-6000模拟渗透胁迫过程中叶片的光合作用、叶绿素荧光、呼吸作用、抗氧化酶活性以及生长状况各参数的变化。研究表明, vde基因缺失使拟南芥植株抽薹和开花时间提前, 渗透胁迫条件下, vde基因缺失突变体较野生型萎蔫程度更高, 成活率更低, 净光合速率(Pn)、气孔导度(Gs)、最大光化学效率(Fv/Fm)、非光化学淬灭系数(NPQ)低于野生型。呼吸作用受到抑制。而且, vde基因的缺失胁迫下部分抗氧化酶(如SOD、APX和CAT)活性提高。结果初步表明, 渗透胁迫下缺失体的过剩光能不能通过热耗散的方式释放, 致使植株的活力下降。关键词:vde基因; 渗透胁迫; 拟南芥; 生理响应
收稿:2015-08-05 修定:2015-10-19
资助:中央高校基本科研业务费专项资金(DL11EA019、 257-2015DA02)、国家自然科学基金(31170569、J1210053)和大学生创新项目。
Corresponding author: SONG Xing-Shun; E-mail: sfandi@163.com; Tel: 0451-82191755
Abstract:
In the present study, vde gene deletion mutant of Arabidopsis, as well as wild-type (Columbia) materials were used to measure photosynthesis, chlorophyll fluorescence parameters (such as net photosynthetic rate, stomatal conductance, maximal photochemical efficiency, non photochemical quenching coefficient), the parameters of the respiration, antioxidant enzyme activity and growth status under PEG-6000 simulated osmotic stress. We found that the deletion of vde gene accelerated the bolting and flowering time. Under osmotic stress, vde mutant had higher wilting levels, lower survival rate compared with wild type. The net photosynthetic rate (Pn), stomatal conductance (Gs), maximum photochemical efficiency (Fv/Fm) and non-photochemical quenching (NPQ) in mutant were lower than that in wild type. Also, its respiration was inhibited to some extents. Moreover, vde gene deletion mutant partially increased the activity of antioxidant enzymes (such as SOD, APX and CAT). These preliminary results indicated that the excess energy of deletions can not be released by the way of heat dissipation, resulting in a decline in plant vigor under osmotic stress.Key words: vde gene; osmotic stress; Arabidopsis; physiological response
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